《植物生理学报》 2013, 49(12): 1333-1338

一种快速、高效花生植株再生体系的建立

马彩霞, 穆国俊, 侯名语, 陈焕英, 崔顺立, 何美敬, 刘立峰*

河北农业大学农学院, 教育部华北作物种质资源实验室, 河北省作物种质资源重点实验室, 河北保定071001

通信作者：刘立峰；E-mail: liulifeng@hebau.edu.cn；Tel: 0312-7520200

摘　要：

快速、高效、重复性好的植株再生体系是转基因育种的基础; 本研究以14份不同花生品种的胚小叶为外植体, 利用不同激素浓度、组合和不同花生基因型筛选最佳芽诱导培养基、伸长培养基和高效再生基因型。结果表明最佳丛生芽诱导培养基为MSB5+0.2 mg·L^-1 NAA+6 mg·L^-1 6-BA, 诱导率为89.50%; 最佳伸长培养基为MSB5+0.2 mg·L^-1 NAA+3 mg·L^-1 6-BA和MSB5+0.2 mg·L^-1 NAA+4 mg·L^-1 6-BA+2 mg·L^-1 GA3交替培养, 每个丛生芽伸长数达到7.24, 时间缩短至3~4周。不同品种再生率的变幅在25.51%~93.01%, 大于80%的品种有‘麻油1-1’、‘弗落蔓生’、‘濮花23号’、‘海花1号’。利用‘弗落蔓生’在15周内得到了生根组培苗。

关键词：花生; 胚小叶; 再生; 丛生芽

收稿：2013-07-18   修定：2013-09-25

资助：现代农业产业技术体系建设专项资金(CARS-14)、高等学校博士学科点专项科研基金(20121302110002)、河北省高等学校科学技术研究重点项目(ZD2010136)和河北省专家出国培训项目。

A Rapid and Efficient Method for Peanut Regeneration

MA Cai-Xia, MU Guo-Jun, HOU Ming-Yu, CHEN Huan-Ying, CUI Shun-Li, HE Mei-Jing, LIU Li-Feng*

Laboratory of Crop Germplasm Resources for Northern China, Ministry of Education, Key laboratory of Crop Germplasm Resources of Hebei Province, College of Agronomy, Agricultural University of Hebei, Baoding, Hebei 071001, China

Corresponding author: LIU Li-Feng; E-mail: liulifeng@hebau.edu.cn; Tel: 0312-7520200

Abstract:

Rapid, efficient and repeatable plant regeneration method is the basis for plant transgenic breeding. In this study, the leaflet of fourteen peanut varieties was used as explants. The effects of different hormone concentration, combination and different peanut genotypes were analyzed to investigate the optimal method of shoots induction medium, elongation medium and high efficiency regeneration genotypes. The results showed that the optimal shoots induction medium was MSB5+0.2 mg·L^-1 NAA+6 mg·L^-1 6-BA with inductivity of 89.50%; optimal elongation media were MSB5+0.2 mg·L^-1 NAA+3 mg·L^-1 6-BA and MSB5+0.2 mg·L^-1 NAA+4 mg·L^-1 6-BA+2 mg·L^-1 GA3 with the alternation of culture. The highest average elongated shoots were 7.24 per explant and the shortest bud elongation period was 3–4 weeks. The regeneration rate of the different genotypes varied from 25.51% to 93.01%. Genotypes with the regeneration rate over 80% were ‘MaYou 1-1’, ‘FuLuoManSheng’, ‘PuHua 23’ and ‘HaiHua 1’. Plantlets were obtained in 15 weeks using genotype of ‘FuLuoManSheng’.

Key words: peanut; leaflet; regeneration; multiple shoots